A new fluorescent 'turn on' probe for rapid detection of biothiols

ÜÇÜNCÜ M., Zeybek H., Karakus E., Ucuncu C., Emrullahoglu M.

SUPRAMOLECULAR CHEMISTRY, cilt.32, sa.12, ss.634-641, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 12
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1080/10610278.2021.1893321
  • Dergi Adı: SUPRAMOLECULAR CHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Chemical Abstracts Core
  • Sayfa Sayıları: ss.634-641
  • Anahtar Kelimeler: Biothiols, ESIPT, electrophilic cyanate, cell imaging
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

We designed and synthesised a novel molecular probe exhibiting high selectivity and sensitivity towards reactive sulphur species (RSS) over other amino acids and biologically relevant species, as well as scrutinised its spectroscopic behaviours under physiological conditions and in living milieu. We used an electrophilic cyanate group as a masking agent to block the excited state intramolecular proton transfer process of 2-(2-cyanato-3-methoxyphenyl)benzo[d]thiazole (HMBT-OCN), which readily hydrolyses to the highly fluorescent structure, 2-(2'-Hydroxy-3'-methoxyphenyl) benzothiazole (HMBT-OH), in the presence of reactive sulphur species. [GRAPHICS] .