Erythropoietin receptor-like immunostaining on human spermatozoa

Tug N., Kilic U., Karateke A., Yilmaz B., Ugur M., Kilic E.

Reproductive BioMedicine Online, cilt.21, sa.5, ss.718-720, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 21 Sayı: 5
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1016/j.rbmo.2010.05.022
  • Dergi Adı: Reproductive BioMedicine Online
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.718-720
  • Anahtar Kelimeler: EPO, EPO-receptor, receptor, spermatozoa
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

This study aimed to demonstrate the presence of erythropoietin (EPO) receptor on spermatozoa. Whole ejaculates of four healthy volunteers were incubated with polyclonal rabbit anti-EPO receptor and subsequently stained with a Cy-3 labelled secondary antibody. Four slides per subject were analysed, no staining was observed in slides incubated with either primary or secondary antibody alone. EPO receptor staining was positive in 92 ± 8% of EPO pre-treated and 91 ± 4% of non-treated sperm cells. The results suggest that spermatozoa express EPO receptor on plasma membrane, which might act to protect these cells from damage after ejaculation. © 2010, Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.