EpCAM-claudin-tetraspanin-modulated ovarian cancer progression and drug resistance

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Tavsan Z., Ayar Kayali H.

CELL ADHESION & MIGRATION, cilt.14, sa.1, ss.57-68, 2020 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 14 Sayı: 1
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1080/19336918.2020.1732761
  • Dergi Adı: CELL ADHESION & MIGRATION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, EMBASE, INSPEC, MEDLINE, Directory of Open Access Journals
  • Sayfa Sayıları: ss.57-68
  • Anahtar Kelimeler: Ovarian cancer, cell adhesion, EpCAM, claudins, tetraspanins, palmitoylation, GENE-EXPRESSION PROFILES, CD44 VARIANT ISOFORMS, E-CADHERIN, EP-CAM, PROTEIN, PALMITOYLATION, KAI1, METASTASIS, OVEREXPRESSION, PROMOTES
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Alterations of cell adhesion are involved in cancer progression, but the mechanisms underlying the progression and cell adhesion have remained poorly understood. Focusing on the complex between EpCAM, claudins and tetraspanins, we described a sequence of events by which of the molecules associate each other in ovarian cancer. The interactions between molecules were evaluated by immunoprecipitations and then immunoblotting. To identify the effects of complex formation on the ovarian cancer progression, the different types of ovarian cancer cell lines were compared. In this study, we report the identification of the EpCAM-claudin-4 or -7-CD82 complex in the ovarian cancer progression and metastasis in vitro. Additionally, we demonstrated palmitoylation and intra- or extra-cellular regions are critically required for the complex formation. These results represent the first direct evidence for the link between the dynamism of cell adhesion molecules and ovarian cancer progression.