Occurrence, genetic characterization and antimicrobial resistance of extended spectrum β-lactamase producing <i>Escherichia coli</i> isolated from Surk samples, a traditional turkish cheese

KÜREKCİ C., Arkadas M., AVŞAR Y. K.

JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION, cilt.10, sa.3, ss.709-714, 2016 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 10 Sayı: 3
  • Basım Tarihi: 2016
  • Doi Numarası: 10.1007/s11694-016-9355-7
  • Dergi Adı: JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.709-714
  • Anahtar Kelimeler: ESBL, E. coli, Surk cheese, CTX-M
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

This study was carried out to provide information regarding the presence of extended spectrum beta-lactamase (ESBL) producing Escherichia coli isolated from Surk cheese samples. For this purpose, ESBL-producing E. coli strains were isolated from 87 Surk samples and isolates were screened for the common ESBL-types, presence of integrons and sul genes via PCR. All ESBL-producing E. coli strains were also allotted to phylogenetic groups and typed by PFGE. Twelve ESBL-producing E. coli (13.8 %) displayed eight distinct PFGE profiles and were exhibited resistance to tetracycline (41.7 %), trimethoprim-sulfamethoxazole (25 %), doxycycline (25 %) and chloramphenicol (16.7 %). Eleven isolates carried the bla (CTX-M-15) gene (n = 7) and bla (CTX-M-15) + bla (TEM-1) genes (n = 4). Eleven isolates carried class1 and 2 integrons (intl1, 9 isolates and intl1 + intl2, two isolates) whereas four isolates carried sul1 (n = 2) and both sul1 + sul2 (n = 2) genes. The current study revealed a high rate of E. coli producing CTX-M-15 enzymes indicating a significant source for this organism in humans.