Enhanced production of manganese peroxidase by Phanerochaete chrysosporium

ÖZTÜRK ÜREK R., Pazarlioglu N. K.

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, cilt.50, sa.6, ss.913-920, 2007 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 50 Sayı: 6
  • Basım Tarihi: 2007
  • Doi Numarası: 10.1590/s1516-89132007000700001
  • Dergi Adı: BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.913-920
  • Anahtar Kelimeler: phanerochaete chrysosporium, manganese dependent peroxidase, Tween 80, Mn2+, phenylmethylsulphonylfloride, WHITE-ROT FUNGI, LIGNINOLYTIC ENZYME-PRODUCTION, LIGNINASE PRODUCTION, SUBMERGED CULTURES, MODIFYING ENZYMES, WOOD DECAY, DEGRADATION, INA-12, ACID, DECOLORIZATION
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Production of manganese-dependent peroxidase (MnP) by the white-rot fungus Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was monitored during growth in different media and growth conditions. The effect of some activators of MnP production, Mn2+, Tween 80, phenylmethylsulphonylfloride (PMSF), oxygen, temperature, pH, glycerol and nitrogen was studied. Supplementing the cultures with Tween 80 (0.05 %, v/v) and Mn2+ (174 mu M) resulted a maximum MnP activity of 356 U/L which was approximately two times higher than that obtained in the control culture (without Tween 80). Decolourisation of Direct Blue 15 and Direct Green 6 (50 mg/L) was also achieved with MnP.