Enhanced production of manganese peroxidase by Phanerochaete chrysosporium
BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY, cilt.50, sa.6, ss.913-920, 2007 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 50 Sayı: 6
- Basım Tarihi: 2007
- Doi Numarası: 10.1590/s1516-89132007000700001
- Dergi Adı: BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.913-920
- Anahtar Kelimeler: phanerochaete chrysosporium, manganese dependent peroxidase, Tween 80, Mn2+, phenylmethylsulphonylfloride
- Dokuz Eylül Üniversitesi Adresli: Evet
Özet
Production of manganese-dependent peroxidase (MnP) by the white-rot fungus Phanerochaete chrysosporium BKM-F-1767 (ATCC 24725) was monitored during growth in different media and growth conditions. The effect of some activators of MnP production, Mn2+, Tween 80, phenylmethylsulphonylfloride (PMSF), oxygen, temperature, pH, glycerol and nitrogen was studied. Supplementing the cultures with Tween 80 (0.05 %, v/v) and Mn2+ (174 mu M) resulted a maximum MnP activity of 356 U/L which was approximately two times higher than that obtained in the control culture (without Tween 80). Decolourisation of Direct Blue 15 and Direct Green 6 (50 mg/L) was also achieved with MnP.