Porous dye affinity beads for albumin separation from human plasma

Akgol S., DEMİR M. N., Denizli A.

JOURNAL OF APPLIED POLYMER SCIENCE, vol.105, no.3, pp.1251-1260, 2007 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 105 Issue: 3
  • Publication Date: 2007
  • Doi Number: 10.1002/app.26178
  • Journal Name: JOURNAL OF APPLIED POLYMER SCIENCE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1251-1260
  • Keywords: dye-affinity chromatography, porous beads, protein purification, albumin, HUMAN SERUM-ALBUMIN, METAL-CHELATE AFFINITY, POLY(2-HYDROXYETHYL METHACRYLATE) BEADS, ATTACHED POLY(EGDMA-HEMA) MICROBEADS, CHITOSAN MICROSPHERES, COLUMN CHROMATOGRAPHY, HOLLOW FIBERS, CONGO-RED, ADSORPTION, PURIFICATION
  • Dokuz Eylül University Affiliated: Yes

Abstract

Porous polymeric beads were obtained by the suspension polymerization of 2-hydroxyethyl methacrylate (HEMA) and ethylene glycol dimethacrylate (EGDMA). Poly(HEMA-EGDMA) beads were characterized by surface area measurements, swelling studies, FTIR, scanning electron microscopy (SEM), and elemental analysis. Poly (HEMA-EGDMA) beads had a specific surface area of 56 m 2/ g. SEM observations showed that the poly(HEMA-EGDMA) beads abounded macropores. Poly(HEMA-EGDMA) beads with a swelling ratio of 55%, and containing different amounts of Reactive Red 120 (9.2-39.8 mu mol/g) were used in the adsorption/desorption of human serum albumin (HSA) from aqueous solutions and human plasma. The nonspecific adsorption of HSA was very low (0.2 mg/g). The maximum HSA adsorption amount from aqueous solution in phosphate buffer was 60.1 mg/g at pH 5.0. Higher HSA adsorption value was obtained from human plasma (up to 95.7 mg/g) with a purity of 88%. The equilibrium monolayer adsorption amount, Q(max) was determined as 172.4 mg/g. The dimensionless separation factor (R-L) value shows that the adsorption behavior of HSA onto the Reactive Red 120 attached poly(HEMA-EGDMA) beads was favorable (0 < RL < 1). Desorption of HSA from Reactive Red 120 attached poly (HEMA-EGDMA) beads was performed using 0.1M Tris/HCl buffer containing 0.5M NaCl. It was observed that HSA could be repeatedly adsorbed and desorbed with Reactive Red 120-attached poly(HEMA-EGDMA) beads without significant loss in the adsorption amount. (c) 2007 Wiley Periodicals, Inc.