Expression of ADAMTS-7 in myocardial dystrophy associated with white muscle disease in lambs

Yumusak N., Yigin A., Polat P. F., Hitit M., Yilmaz R.

POLISH JOURNAL OF VETERINARY SCIENCES, cilt.21, sa.1, ss.119-126, 2018 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 21 Sayı: 1
  • Basım Tarihi: 2018
  • Doi Numarası: 10.24425/119029
  • Dergi Adı: POLISH JOURNAL OF VETERINARY SCIENCES
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.119-126
  • Anahtar Kelimeler: ADAMTS-7, myocardial dystrophy, lamb, white muscle disease, OLIGOMERIC MATRIX PROTEIN, EXTRACELLULAR-MATRIX, CELL MIGRATION, METALLOPROTEINASES, INFARCTION, OSTEOARTHRITIS, DISINTEGRIN, CALF
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

The aim of the present study was to investigate the role of ADAMTS-7 gene in the pathogenesis of myocardial dystrophy associated with white muscle disease (WMD) in lambs. A total of 217 cardiac tissue samples from lambs with WMD were used in the study. Histopathological sections of the samples were stained with hematoxylin-eosin (HE) and examined using Western- blot, real-time PCR (RT-PCR) and immunohistochemistry for ADAMTS-7 gene expression, and the findings were statistically evaluated. Histopathological examinations revealed fibrosis associated with hyalinization, necrosis and granular calcifications in cardiomyocytes. Western blot and RT-PCR showed a statistically significant upregulation of ADAMTS-7 (p<0.05) (p<0.05). Immunohistochemical analyses showed that immunopositive cell numbers significantly high for ADAMTS-7 (p<0.05). The study has revealed that ADAMTS-7 gene is significantly expressed in myocardial dystrophy associated with WMD in addition to its role in the pathogenesis of this disease.