Optimization of media composition for hydrogen gas production from hydrolyzed wheat starch by dark fermentation

Oztekin R., Kapdan İ., Kargi F., Argun H.

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, cilt.33, sa.15, ss.4083-4090, 2008 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 33 Sayı: 15
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1016/j.ijhydene.2008.05.052
  • Dergi Adı: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.4083-4090
  • Anahtar Kelimeler: biohydrogen, Box-Wilson experiment design, dark fermentation, acid-hydrolyzed wheat powder, N/C, P/C, Fe(II)/C ratios, BIOHYDROGEN PRODUCTION, H-2 PRODUCTION, RHODOBACTER-SPHAEROIDES, CLOSTRIDIUM-BUTYRICUM, MIXED CULTURE, ACETATE, RATIO
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Effects of N/C, P/C and Fe(II)/C ratios in fermentation medium on biohydrogen production by dark fermentation of acid-hydrolyzed wheat starch was investigated. The powdered wheat was autoclaved at pH = 3 and 90 degrees C for 15 min and the resulting sugar solution was fermented after external addition of N, P and Fe(II) to overcome nutrient limitations. Box-Wilson statistical experiment design was used by considering the N/C (0-0.05, w w(-1)), P/C (0-0.02) and Fe(II)/C (0-0.03) ratios as the independent variables while the hydrogen yield and specific hydrogen production rate (SHPR) were the objective functions to be optimized. A quadratic response function was used to correlate the response functions with the independent variables. Low levels of the variables (N/C < 0.02, P/C < 0.01, Fe(II)/C < 0.01) resulted in low hydrogen yield and SHPR due to nutrient limitations and high levels of nutrients caused inhibitions. The optimum conditions yielding the maximum hydrogen yield (Y = 2.84 mol H-2 mol(-1) glucose) were N/C = 0.02, P/C = 0.008 and Fe(II)/C 0.015. The maximum SHPR (96 mL H-2 g(-1) biomass h(-1)) was obtained at N/C = 0.025, P/C = 0.008 and Fe(II)/C 0.015 (w w-1). (C) 2008 International Association for Hydrogen Energy. Published by Elsevier Ltd. All rights reserved.