Grape seed extract has superior beneficial effects than vitamin E on oxidative stress and apoptosis in the hippocampus of streptozotocin induced diabetic rats


Creative Commons License

Yonguc G. N., Dodurga Y., Adıgüzel E., Gundogdu G., Küçükatay V., Özbal S., ...Daha Fazla

GENE, cilt.555, sa.2, ss.119-126, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 555 Sayı: 2
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1016/j.gene.2014.10.052
  • Dergi Adı: GENE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.119-126
  • Anahtar Kelimeler: Diabetes, Grape seed extract, Vitamin E, Hippocampus, Oxidative stress, Apoptosis, NF-KAPPA-B, GLUCOSE-INDUCED APOPTOSIS, RETINAL GANGLION-CELLS, PROANTHOCYANIDIN EXTRACT, COGNITIVE DYSFUNCTION, PROCYANIDIN EXTRACT, CEREBRAL-ISCHEMIA, SCIATIC-NERVE, DNA-DAMAGE, PC12 CELLS
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

We aimed to investigate the effects of grape seed extract (GSE) and vitamin E (Vit E) on oxidative stress and apoptosis in the hippocampus of streptozotocin-induced diabetic rats. In Control, Diabetic, and Diabetic treated with GSE (Diabetic + GSE) and vitamin E (Diabetic + Vit E) groups, oxidative stress index (OSI), TUNEL staining and Bcl-2, Bcl-XL, Bax, caspase-3, -9, and -8, Cyt-c, TNF-alpha, and NF-kappa B gene expressions were evaluated. OSI was significantly increased in the plasma and hippocampus of the Diabetic compared to Control group and decreased in Diabetic + GSE and Diabetic + Vit E groups compared to Diabetic. TUNEL positive neurons significantly increased in the hippocampus of the Diabetic group compared to Control and decreased in Diabetic + GSE (more prominently) and Diabetic + Vit E groups compared to Diabetic. In the hippocampus of the Diabetic group, Bcl-2 and Bcl-XL gene expressions were significantly decreased; Bax, caspase-3, -9, and -8, Cyt-c, TNF-alpha, and NF-kappa B gene expressions were significantly increased compared to Control. In Diabetic + GSE and Diabetic + Vit E groups, Bcl-2 gene expressions were significantly increased; Bcl-XL gene expressions did not differ compared to the Diabetic group. The expression of Bax, caspase-3, -9, and -8, Cyt-c, TNF-alpha, and NF-kappa B genes in the Diabetic + GSE group and the expression of caspase-3 and -9, TNF-alpha, and NF-kappa B genes in the Diabetic + Vit E group were significantly decreased compared to Diabetic. In conclusion, GSE (more prominently) and vitamin E decreased oxidative stress and neuronal apoptosis occurring in the hippocampus of diabetic rats. (C) 2014 Elsevier B.V. All rights reserved.