Grape seed extract has superior beneficial effects than vitamin E on oxidative stress and apoptosis in the hippocampus of streptozotocin induced diabetic rats


Yonguc G. N., Dodurga Y., Adıgüzel E., Gundogdu G., Küçükatay V., Özbal S., ...More

GENE, vol.555, no.2, pp.119-126, 2015 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 555 Issue: 2
  • Publication Date: 2015
  • Doi Number: 10.1016/j.gene.2014.10.052
  • Journal Name: GENE
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.119-126
  • Keywords: Diabetes, Grape seed extract, Vitamin E, Hippocampus, Oxidative stress, Apoptosis, NF-KAPPA-B, GLUCOSE-INDUCED APOPTOSIS, RETINAL GANGLION-CELLS, PROANTHOCYANIDIN EXTRACT, COGNITIVE DYSFUNCTION, PROCYANIDIN EXTRACT, CEREBRAL-ISCHEMIA, SCIATIC-NERVE, DNA-DAMAGE, PC12 CELLS
  • Dokuz Eylül University Affiliated: Yes

Abstract

We aimed to investigate the effects of grape seed extract (GSE) and vitamin E (Vit E) on oxidative stress and apoptosis in the hippocampus of streptozotocin-induced diabetic rats. In Control, Diabetic, and Diabetic treated with GSE (Diabetic + GSE) and vitamin E (Diabetic + Vit E) groups, oxidative stress index (OSI), TUNEL staining and Bcl-2, Bcl-XL, Bax, caspase-3, -9, and -8, Cyt-c, TNF-alpha, and NF-kappa B gene expressions were evaluated. OSI was significantly increased in the plasma and hippocampus of the Diabetic compared to Control group and decreased in Diabetic + GSE and Diabetic + Vit E groups compared to Diabetic. TUNEL positive neurons significantly increased in the hippocampus of the Diabetic group compared to Control and decreased in Diabetic + GSE (more prominently) and Diabetic + Vit E groups compared to Diabetic. In the hippocampus of the Diabetic group, Bcl-2 and Bcl-XL gene expressions were significantly decreased; Bax, caspase-3, -9, and -8, Cyt-c, TNF-alpha, and NF-kappa B gene expressions were significantly increased compared to Control. In Diabetic + GSE and Diabetic + Vit E groups, Bcl-2 gene expressions were significantly increased; Bcl-XL gene expressions did not differ compared to the Diabetic group. The expression of Bax, caspase-3, -9, and -8, Cyt-c, TNF-alpha, and NF-kappa B genes in the Diabetic + GSE group and the expression of caspase-3 and -9, TNF-alpha, and NF-kappa B genes in the Diabetic + Vit E group were significantly decreased compared to Diabetic. In conclusion, GSE (more prominently) and vitamin E decreased oxidative stress and neuronal apoptosis occurring in the hippocampus of diabetic rats. (C) 2014 Elsevier B.V. All rights reserved.