Preparation of Pt-TiS2 nanocomposite based amperometric glucose biosensor
Nanomedicine: Nanotechnology, Biology, and Medicine, cilt.74, 2026 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 74
- Basım Tarihi: 2026
- Doi Numarası: 10.1016/j.nano.2026.102937
- Dergi Adı: Nanomedicine: Nanotechnology, Biology, and Medicine
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, BIOSIS, Chemical Abstracts Core, Compendex, EMBASE, INSPEC, MEDLINE
- Anahtar Kelimeler: Biosensor, Glucose, Glucose oxidase, Pt-TiS2 nanocomposite
- Dokuz Eylül Üniversitesi Adresli: Evet
Özet
In this study, an amperometric biosensor was developed using a glucose oxidase-functionalized Pt–TiS₂ nanocomposite for the sensitive and accurate detection of glucose. Pt nanoparticles (5–10 nm) were uniformly distributed on layered TiS₂ nanosheets (~100 nm length), ensuring high surface area and efficient electron transfer. The biosensor exhibited a linear detection range of 0.01–1 mM with a low detection limit of 0.018 μM and a quantification limit of 0.048 μM. The calculated sensitivity was determined to be 5.7392 μA·mM−1·cm−2, and the apparent Michaelis-Menten constant (Km) was 0.138 mM, indicating strong enzyme-substrate affinity. The biosensor demonstrated exceptional selectivity against prevalent interferents, exhibiting high reproducibility (RSDs ranging from 0.20% to 4.20%) and notable reusability, with a mere 14.49% signal loss over the span of 10 cycles. The Pt–TiS₂/GOx biosensor exhibited 74.8% of its activity after a 60-day storage period at a temperature of 4 °C. In commercial serum samples, recovery rates were 106.9%, 105.7%, and 102.2% for 500, 1000, and 5000 μM glucose, respectively. The results obtained demonstrate the considerable potential of the Pt–TiS₂/GOx biosensor for accurate and reliable glucose monitoring in clinical settings.