Relationship between morphology, rheology and polygalacturonase production by Aspergillus sojae ATCC 20235 in submerged cultures

GÖĞÜŞ BAĞIŞ N., Tari C., Oncu Ş., Unluturk S., Tokatli F.

BIOCHEMICAL ENGINEERING JOURNAL, cilt.32, sa.3, ss.171-178, 2006 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 32 Sayı: 3
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1016/j.bej.2006.09.023
  • Dergi Adı: BIOCHEMICAL ENGINEERING JOURNAL
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.171-178
  • Anahtar Kelimeler: Aspeigillus sojae, Rhizopus oryzae, polygalacturonase, submerged fermentation, fungal morphology, rheology, PECTINOLYTIC ENZYME, FUNGAL MORPHOLOGY, OPTIMIZATION, FERMENTATION, PARAMETERS, PELLETS, GROWTH, NIGER, FORM
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

A full factorial statistical design, with the factors of, two taxonomically different strains, seven types of seed culture formulations (slants) and two types of fermentation media were used to investigate the effect of these parameters on the morphology and polygalacturonase production. The rheology of the final fermentation medium was analyzed and appropriate mathematical model was applied to calculate suspension viscosity. It was found that most fermentation broths showed non-Newtonian flow behavior. According to statistical analysis, factors of strain types and fermentation media and the interaction between them were found significant on the enzyme activity. The effect of seed culture formulations (slants) were found insignificant at the significance level of 1%. Interaction of slants with strain types and fermentation media were also found insignificant. Considering the morphology of the final culture, Aspergillus sojae with the desired pellet morphology in a complex media, inoculated with a seed culture prepared from molasses resulted in maximum polygalacturonase enzyme activity (0.2 U/ml) and lowest suspension viscosity with a broth rheology close to Newtonian flow behavior. (c) 2006 Elsevier B.V. All rights reserved.