Kalsiyum fruktoboratın SIRT1 enzim aktivitesi üzerindeki konsantrasyona bağlı inhibitör etkisi: Borik asit ile karşılaştırmalı bir analiz

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Çil E. N., Soysal Y.

JOURNAL OF BASIC AND CLINICAL HEALTH SCIENCES, cilt.10, ss.397-402, 2026 (ESCI, TRDizin)

Özet

ABSTRACT

Purpose: SIRT1 functions in regulatory mechanisms including cellular aging, apoptosis, glucose and lipid

metabolism, oxidative stress and inflammation. Studies show that SIRT1 inhibitors may affect various

types of cancer and metabolic disorders, making SIRT1 an important therapeutic target. Boric acid is an

inorganic boron compound with antimicrobial, antifungal and potential anticancer effects. Calcium

fructoborate, another boron compound found in vegetables and fruits, has anti-inflammatory, antioxidant

and anti-tumor effects. This study aimed to investigate the inhibitory potential of calcium fructoborate on

SIRT1 activity and to evaluate its possible use as a chemical tool for future studies targeting SIRT1

regulation.

Material and Methods: In this study, the effect of calcium fructoborate on SIRT1 activity was investigated

by a fluorescence-based method using human recombinant SIRT1.

Results: Calcium fructoborate inhibited SIRT1 activity in a concentration-dependent manner. Calcium

fructoborate significantly inhibited SIRT1 activity by 42% at a concentration of 20 mM (p < 0.001). Boric

acid did not produce a statistically significant or consistent concentration-dependent change in SIRT1

activity within the tested concentration range.

Conclusion: These preliminary cell-free findings suggest that calcium fructoborate inhibits SIRT1 activity,

indicating its potential as a chemical tool for studying the biological functions of SIRT1. However, the

biological relevance of this effect and its potential therapeutic implications require confirmation through

further studies in cellular and in vivo models.