Boron nitride nanosheet modified amperometric biosensor for uric acid determination

Öndeș B., SUNNA Ç., Kilimci U., Uygun M., Uygun D. A.

Microchemical Journal, cilt.194, 2023 (SCI-Expanded) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 194
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1016/j.microc.2023.109240
  • Dergi Adı: Microchemical Journal
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Food Science & Technology Abstracts, Index Islamicus, Veterinary Science Database
  • Anahtar Kelimeler: Biosensor, Boron nitride, Uric acid, Uricase
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

In this study, enzyme-modified boron nitride nanosheets as a catalytic two-dimension material for the sensor application developed for the first time. In this developed system, uricase interacted with B-N groups by the two-dimensional structure of the boron nitride layer. In this way, a unique catalytic structure was created by binding uricase to B-N groups, and this structure was protected by NafionTM. The developed enzyme-based biosensor exhibited a wide linear detection range (5–3000 µM), and a low limit of detection and quantitation values (0.14 µM and 0.46 µM, respectively) for uric acid. Also, the developed system performed good reproducibility, reusability, high selectivity, sensitivity, low Km value, and excellent shelf-life properties. Furthermore, this enzyme-based biosensor has successfully identified uric acid in commercial human serum with a high recovery rate. It has promised rapid, high sensitivity and real serum applications to determine the level of uric acid.