Evaluation of Tc-99m-Pheophorbide-a use in infection imaging: A rat model

Ocakoglu K., Bayrak E., Onursal M., YILMAZ O., Lambrecht F. Y., Holzwarth A. R.

APPLIED RADIATION AND ISOTOPES, vol.69, no.9, pp.1165-1168, 2011 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 69 Issue: 9
  • Publication Date: 2011
  • Doi Number: 10.1016/j.apradiso.2011.03.047
  • Journal Name: APPLIED RADIATION AND ISOTOPES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1165-1168
  • Keywords: Pheophorbide-a, S. aureus, Tc-99m, Infection, Inflammation, PHOTODYNAMIC THERAPY, NA-PHEOPHORBIDE, BIODISTRIBUTION
  • Dokuz Eylül University Affiliated: Yes

Abstract

This study aims to prepare (99m)Technetium Pheophorbide-a (Tc-99m-PH-A) complex and evaluate its efficiency as an infection imaging agent. First, PH-A was obtained from Spirulina maxima algae, and the product compound was confirmed using H-1 NMR and MS (ESI) methods. The PH-A was then labeled with Tc-99m using the tin chloride method and its biological efficacy as a potential radiotracer for Staphylococcus aureus (S. aureus) infection was evaluated in bacterially infected and sterile inflamed rats. The radiochemical stability of the Tc-99m-PH-A in human serum was determined by thin-layer radiochromatography (TLRC). The radiochemical purity was 87 +/- 3.2% and remained constant at more than 80 +/- 0.1% even in serum for 120 min after radiolabeling.