Evaluation of Tc-99m-Pheophorbide-a use in infection imaging: A rat model
APPLIED RADIATION AND ISOTOPES, cilt.69, sa.9, ss.1165-1168, 2011 (SCI-Expanded, Scopus)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 69 Sayı: 9
- Basım Tarihi: 2011
- Doi Numarası: 10.1016/j.apradiso.2011.03.047
- Dergi Adı: APPLIED RADIATION AND ISOTOPES
- Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
- Sayfa Sayıları: ss.1165-1168
- Anahtar Kelimeler: Pheophorbide-a, S. aureus, Tc-99m, Infection, Inflammation
- Dokuz Eylül Üniversitesi Adresli: Evet
Özet
This study aims to prepare (99m)Technetium Pheophorbide-a (Tc-99m-PH-A) complex and evaluate its efficiency as an infection imaging agent. First, PH-A was obtained from Spirulina maxima algae, and the product compound was confirmed using H-1 NMR and MS (ESI) methods. The PH-A was then labeled with Tc-99m using the tin chloride method and its biological efficacy as a potential radiotracer for Staphylococcus aureus (S. aureus) infection was evaluated in bacterially infected and sterile inflamed rats. The radiochemical stability of the Tc-99m-PH-A in human serum was determined by thin-layer radiochromatography (TLRC). The radiochemical purity was 87 +/- 3.2% and remained constant at more than 80 +/- 0.1% even in serum for 120 min after radiolabeling.