Sensitive Electrochemical Determination of Hesperetin at Gold Nanoparticles/Poly Luminol/Graphene Oxide/Glassy Carbon Electrode

Zade S., KOÇAK Ç. C., KOÇAK S.

Analytical Letters, 2025 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1080/00032719.2025.2555906
  • Dergi Adı: Analytical Letters
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Food Science & Technology Abstracts, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: Electrochemical sensor, gold nanoparticles, hesperetin, linear sweep voltammetry (LSV), polyluminol
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

An electrochemical sensor platform was designed by sequentially modifying a glassy carbon electrode (GCE) with graphene oxide (GO), poly(luminol) (PLu), and gold nanoparticles (AuNPs). GO was synthesized using a modified Brodie method followed by luminol electropolymerization and AuNP deposition. The surface characteristics of the fabricated Au/PLu/GO/GCE electrode were examined using scanning electron microscopy and electrochemical impedance spectroscopy. The prepared composite electrode was successfully applied to the electrochemical determination of hesperetin, a naturally occurring flavonoid with a wide range of important applications. The proposed sensor exhibited a wide linear range (0.02–100.0 µmol L−1), low detection limit (6.1 nmol L−1), and a quantification limit of 18 nmol L−1. Au/PLu/GO/GCE demonstrated high repeatability, reproducibility, and maintained 94% of its initial signal over 15 days. Interference studies showed that the presence of commonly encountered electroactive species had a minimal effect on the oxidation signal of hesperetin. Successful analyses were performed on real orange peel samples using the standard addition method, and the obtained recovery results confirmed the sensor’s reliability for application in complex matrices.