Butyrate generation during the fermentation of biohydrogen as a green chemical


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Karakaya G., Özmıhçı S.

11. Global Conference on Global Warming, İstanbul, Turkey, 14 - 16 June 2023, vol.00, no.0

  • Publication Type: Conference Paper / Full Text
  • Volume: 00
  • City: İstanbul
  • Country: Turkey
  • Dokuz Eylül University Affiliated: Yes

Abstract

Trace element addition was optimized for hydrogen and butyric acid production using a three-level Box Behnken Design of the response surface methodology. With sodium chloride, sodium thiosulphate, and minerals (Fe, Zn, Cu, and Ni) as variables, seventeen runs were planned. The model has a high R2 of 96% and a P value of 0.03 and fits a cubic model. The generation of butyrate was significantly impacted by certain of the interacting variables. The maximal butyrate concentration produced by the lactose substrate when 10 g/L of lactose was used (4.64 g/L) was extremely close to the expected value. The greatest BA/AA ratio (2,65 g/g) found from the experimental setup resulted in a cumulative hydrogen formation of 178.8 mL, a Yp/s of 3.6 mmolH2/mmol lactose, and an HPR of 2.4 mL/g/h.