11. Global Conference on Global Warming, İstanbul, Turkey, 14 - 16 June 2023, vol.00, no.0
Trace element addition was optimized for
hydrogen and butyric acid production using a three-level Box Behnken Design of
the response surface methodology. With sodium chloride, sodium thiosulphate,
and minerals (Fe, Zn, Cu, and Ni) as variables, seventeen runs were planned.
The model has a high R2 of 96% and a P value of 0.03 and fits a
cubic model. The generation of butyrate was significantly impacted by certain
of the interacting variables. The maximal butyrate concentration produced by
the lactose substrate when 10 g/L of lactose was used (4.64 g/L) was extremely
close to the expected value. The greatest BA/AA ratio (2,65 g/g) found from the
experimental setup resulted in a cumulative hydrogen formation of 178.8 mL, a
Yp/s of 3.6 mmolH2/mmol lactose, and an HPR of 2.4 mL/g/h.