Antiproliferative and newly attributed apoptotic activities from an invasive marine alga: Caulerpa racemosa var. cylindracea

Çavaş L., Başbınar Y., Yurdakoç M. K., Olgun N.

JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY, cilt.339, sa.1, ss.111-119, 2006 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 339 Sayı: 1
  • Basım Tarihi: 2006
  • Doi Numarası: 10.1016/j.jembe.2006.07.019
  • Dergi Adı: JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.111-119
  • Anahtar Kelimeler: antiproliferation, apoptosis, cancer, Caulerpa racemosa var. cylindracea neuroblastoma, WOUND-ACTIVATED TRANSFORMATION, SEA-URCHIN, TAXIFOLIA, CHLOROPHYTA, PROTEIN
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Caulerpa racemosa (Forsskal) is a green marine alga which spreads from tropical to warm-water regions. Due to having invasive capacity C racemosa var. cylindracea is a well-known biological pollution in Mediterranean Sea. One of the most important secondary metabolites of C. racemosa is Caulerpenyne (CPN). In the present study, antiproliferative and apoptotic effects of C. racemosa var. cylindracea extract and purified CPN on two well-known neuroblastoma cell lines, SHSY5Y and Kelly, are investigated. The antiproliferative and, additionally, newly attributed apoptotic effects of both C. racemosa var. cylindracea extract and purified CPN on SHSY5Y and Kelly cell lines have been shown in the present study. IC50 values are 0.59 +/- 0.06; 1.06 0.23 g wet alga/methanol and 5.64 +/- 0.09; 6.02 +/- 0.09 mu M CPN for C. racemosa var. cylindracea extract and purified CPN on SHSY5Y and Kelly cell lines, respectively. Percentages of apoptotic cells of SHSY5Y and Kelly in 0, 0.1 and 1 mu M CPN conditions were 1.00 +/- 0.71, 3.00 +/- 0.71 and 49.40 +/- 3.78, 39.60 +/- 6.19 and 78.00 +/- 2.74, 69.40 +/- 3.78, respectively. In conclusion, the present study shows the antiproliferative effect of C. racemosa var. cylindracea extract and newly attributed apoptotic effects of C. racemosa var. cylindracea this extract. Compared to other alkylating anticancer drugs, CPN and also C. racemosa var. cylindracea extract might be considered as an alternative native source of antitumor drugs. Inasmuch as both C. racemosa extract and CPN have shown both antiproliferative and apoptotic effects on SHSY5Y and Kelly cell lines, the CPN and CPN derivatives might be considered as multifunctional agents in cell metabolism. (c) 2006 Elsevier B.V. All rights reserved.