Effects of dark/light bacteria ratio on bio-hydrogen production by combined fed-batch fermentation of ground wheat starch

Kargi F., ÖZMIHÇI S.

BIOMASS & BIOENERGY, vol.34, no.6, pp.869-874, 2010 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 6
  • Publication Date: 2010
  • Doi Number: 10.1016/j.biombioe.2010.01.031
  • Journal Name: BIOMASS & BIOENERGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.869-874
  • Keywords: Bio-hydrogen, Combined fermentation, Dark fermentation, Fed-batch operation, Light fermentation, Wheat starch, CONTINUOUS BIOHYDROGEN PRODUCTION, DARK FERMENTATION, CLOSTRIDIUM-BUTYRICUM, RHODOBACTER-SPHAEROIDES, LIGHT FERMENTATION, H-2 PRODUCTION, MIXED CULTURE, WASTE, COCULTURES, SUBSTRATE
  • Dokuz Eylül University Affiliated: Yes

Abstract

Bio-hydrogen production by combined dark and light fermentation of ground wheat starch was investigated using fed-batch operation. Serum bottles containing heat-treated anaerobic sludge and a mixture of Rhodobacter sp. was fed with a medium containing 20 g dm(-3) wheat powder (WP) at a constant flow rate. The system was operated at different initial dark/light biomass ratios (D/L). The optimum D/L ratio was 1/2 yielding the highest cumulative hydrogen (1548 cm(3)), yield (65.2 cm(3) g (1) starch), and specific hydrogen production rate (5.18 cm(3) g (1) h (1)). Light fermentation alone yielded higher hydrogen production than dark fermentation due to fermentation of volatile fatty acids (VFAs) to H(2) and CO(2). The lowest hydrogen formation was obtained with D/L ratio of 1/1 due to accumulation of VFAs in the medium. (C) 2010 Elsevier Ltd. All rights reserved.