Curcumin-Functionalized Ag and ZnO Nanoparticles: A Nanotherapeutic Approach for Treating Infections in Diabetic Wounds

Dolatyari M., Rostami P., Rostami M., Rostami A., Mirtagioglu H.

Bioengineering, vol.12, no.10, 2025 (SCI-Expanded, Scopus)

  • Publication Type: Article / Article
  • Volume: 12 Issue: 10
  • Publication Date: 2025
  • Doi Number: 10.3390/bioengineering12101090
  • Journal Name: Bioengineering
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, INSPEC, Directory of Open Access Journals
  • Keywords: wound healing, nanoparticles, molecular docking, curcumin, dressing pad, ointment
  • Dokuz Eylül University Affiliated: Yes

Abstract

Chronic wounds, such as diabetic ulcers, remain a significant clinical challenge due to high infection risk and delayed healing. This study presents a comprehensive evaluation of a novel wound dressing incorporating curcumin-functionalized silver–zinc oxide (Ag-ZnO) nanoparticles. The formulation was rationally designed based on molecular docking simulations that identified curcumin as a high-affinity ligand for Staphylococcus aureus Protein A. The synthesized nanoparticles demonstrated potent, broad-spectrum antibacterial activity, achieving complete inhibition of multidrug-resistant pathogens, including MRSA, within 60 s. A critical comparative assessment, incorporating an unloaded Ag-ZnO nanoparticle control group, was conducted in both a rabbit wound model and a randomized clinical trial (n = 75 patients). This design confirmed that the enhanced wound-healing efficacy is specifically attributable to the synergistic effect of curcumin combined with the nanoparticles. The curcumin-loaded Ag-ZnO treatment group showed a statistically significant reduction in healing time compared to both standard care and unloaded nanoparticle controls (e.g., medium wounds: 19.6 days vs. 90.6, p < 0.001). These findings demonstrate that curcumin-functionalized Ag-ZnO nanoparticles offer a safe and highly effective therapeutic strategy, providing robust antibacterial action and significantly accelerated wound healing.