Focusing on a new biohydrogen production strategy (HS-HS) using Chlamydomonas reinhardtii mutants: The effects of mutation points on biohydrogen production capacity

Yarkent Ç., Oral İ., Öncel D., Oncel S. S.

International Journal of Hydrogen Energy, 2023 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Publication Date: 2023
  • Doi Number: 10.1016/j.ijhydene.2023.02.034
  • Journal Name: International Journal of Hydrogen Energy
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Artic & Antarctic Regions, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, Environment Index, INSPEC
  • Keywords: Biohydrogen, C. reinhardtii mutants, Chlamydomonas reinhardtii, HS, Photobioreactor
  • Dokuz Eylül University Affiliated: Yes


In this study, we investigated biohydrogen production potentials of Chlamydomonas reinhardtii strains as CC124, D1 mutants, PGRL1 mutants, mitochondrial mutant AOX, and control strains as WT and T-1. Among all cultures, D240-41, PGRL1xΔnd4 Véro and PGRL1 mt (+) mutants produced the highest amount biohydrogen as 150 ± 2, 170 ± 2 and 140 ± 2 mL H2 L−1, respectively during TAP-TAP (-S) strategy. Then, the three biohydrogen production strategies (TAP-TAP (-S), TAP-HS, and HS-HS) were compared to each other by using the CC124 strain. The culture was capable of producing biohydrogen during the performing TAP-TAP (-S), and HS-HS strategies. The voltage differences were observed at 225 ± 1 mV and 24 ± 1 mV, respectively. Then, D240-41, PGRL1xΔnd4 Véro and PGRL1 mt (+) cultures were evaluated according to HS-HS strategy. The D240-41 strain caused the highest difference in the voltage measurement, which was attained at 80 ± 1 mV. As a result, all selected strains were able to produce hydrogen during this new HS-HS strategy.