The aim of this study was to evaluate the distributions of yeast species according to the years and to detect the emerging pathogens in intensive care units (ICU). For this purpose, yeast isolation rates were detected retrospectively, in the following time periods: Period I: April-December 2001; period II: January-December 2002; period III: January-December 2003; period IV: January-December 2004. A total of 490 yeast isolates recovered from 462 clinical specimens obtained from 360 different ICU patients were investigated during these periods. Urine (62.1%), blood (13.6%) and tracheal aspirate (8.7%) samples were detected as the most common specimens. Of these isolates, 53.3% were identified as Candida albicans, 14.5% as C.tropicalis, 12.2% as C.glabrata, 6.5% as C.parapsilosis, 4.5% as Trichosporon spp., 3.9% as C.kefyr, 1.6% as C.krusei, 1.4% as Geotrichum candidum and 2.1% as other Candida species. The isolation rates of C.albicans in the periods of I to IV were found as 47.7%, 55.5%, 41.7% and 62.4%, respectively. The decrease between the second and third periods, and increase between third and fourth periods were statistically significant (x2=4.15, p=0.04 and x2=8.32, p=0.004). C.glabrata was the second most common species in the first and second periods (14.8% and 15.5%, respectively), followed by C.tropicalis (12.5% and 10.0%, respectively), however this array has changed in the third and fourth periods (C.tropicalis was the second with the rates of 16.7% and 16.8%, while C.glabrata placed in the third line with the rates of 14.8% and 7.6%, respectively), It was concluded that C.albicans has still been the most frequent species among yeast isolates of ICU's in our hospital; however, the incidence of non-albicans species like C.glabrata and C.tropicalis has increased.