Cloning and expression of a novel human galectin cDNA, predominantly expressed in placenta

Yang Q., Ying K., Yuan H., Chen J., Meng X., Wang Z., ...More

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, vol.1574, no.3, pp.407-411, 2002 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 1574 Issue: 3
  • Publication Date: 2002
  • Doi Number: 10.1016/s0167-4781(01)00319-0
  • Journal Name: BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.407-411
  • Keywords: galectin, placenta, nuclear localization signal, unigene, NEMATODE CAENORHABDITIS-ELEGANS, CARBOHYDRATE-RECOGNITION DOMAIN, GALACTOSIDE-BINDING LECTINS, LEYDEN CRYSTAL PROTEIN, NUCLEAR-LOCALIZATION, 32-KDA GALECTIN, ANIMAL LECTINS, SPLICE SITES, EVOLUTION, FAMILY
  • Dokuz Eylül University Affiliated: No

Abstract

A novel human galectin cDNA (PPL13) was isolated by screening a human 18-week fetal brain library. The mRNA was predominantly expressed in placenta, while the expression of it was not or barely detectable in he-art, brain, lung, liver, skeletal muscle, kidney, and pancreas by Northern blot. COS-7 cells transfected with cDNA encoding human PPL13 sequestered the protein in nuclei although it lacked any known nuclear localization signal. STS of Unigene Hs. 24236 placed the cDNA to human chromosome 19q13.2. (C) 2001 Elsevier Science B.V. All rights reserved.