Akademik Veri Yönetim Sistemi
DR. MİRZA GÖKGÖL INTERNATIONAL PLANT GENETIC RESOURCES SYMPOSIUM, İzmir, Türkiye, 6 - 09 Kasım 2023, ss.67, (Özet Bildiri)
DNA isolation is one of the fundamental steps in various molecular analyses.
The quantity and purity of the obtained DNA in this step are crucial considerations for studies
utilizing molecular analyses. Research conducted in maize genetic studies has demonstrated
that molecular analyses can be performed on tissue samples taken from the endosperm part of
the seed, yielding reliable results. However, there are still divergent recommendations regarding
the number and method of seed sampling for maize populations showing genetic diversity
through open pollination. This study aims i) to compare the quantity and quality of DNA
isolated from seed tissue samples depending on the number of samples and ii) to monitor the
viability status in the sampled seeds. Eleven local maize landraces and two standard lines (B73
and Mo17) were employed as materials in the study. A total of 30 seed samples per genotype
were examined by forming 4 subgroups for each genotype as single, 10, 20 and 30 seeds using
the chipping technique. DNA isolation was performed on these samples using the CTAB
method. The DNA contents and purities of the samples were determined using a Nano-Drop
device. According to the research findings, the DNA quantities of the samples ranged from 2.2
ng to 1243 ng. Purity values ranged between 1.41 and 2.03 (A260/A280). Although the DNA
purity obtained from single seed samples was lower than the other groups, the bulked samples
showed similarity in terms of DNA purity. The germination rates of the sampled seeds ranged
from 55% to 100%. It was determined that this sampling method might have risk in smallseeded populations. DNA samples obtained from the study will undergo genetic similarity
analyses based on SSR markers, and the results will be shared with researchers working in this
field