Effect of different culture media on isolation and differentiation of dendritic cells

Yılmaz Göler A. M., Altundag E. M., Gedik G., Kocturk S., Yalçın A. S., Taga Y.

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI, vol.40, no.6, pp.440-447, 2015 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 40 Issue: 6
  • Publication Date: 2015
  • Doi Number: 10.1515/tjb-2015-0031
  • Journal Name: TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED)
  • Page Numbers: pp.440-447
  • Keywords: Dendritic cells, peripheral blood mononuclear cells, serum-free media, CD209a, IL-10, IL-12, PERIPHERAL-BLOOD, LANGERHANS CELLS, IN-VITRO, HEMATOPOIETIC PROGENITORS, BONE-MARROW, T-CELLS, DC-SIGN, GENERATION, MACROPHAGES, TOLERANCE
  • Dokuz Eylül University Affiliated: Yes

Abstract

Objective: Dendritic cells (DCs) are members of the mammalian immune system and are considered to be the most powerful antigen presenting cells. They are responsible for the induction of T-cells or T-cell dependent immunity and tolerance. In this study we have investigated the effect of different serum supplements on generation and yield of mature dendritic cells isolated from peripheral blood mononuclear cells.