Multi-Walled Carbon Nanotubes and Gold Nanorod Decorated Biosensor for Detection of microRNA-126

TOPKAYA ÇETİN S. N., Turunc E., ÇETİN A. E.

ELECTROANALYSIS, vol.33, no.9, pp.2078-2086, 2021 (SCI-Expanded, Scopus) identifier identifier

  • Publication Type: Article / Article
  • Volume: 33 Issue: 9
  • Publication Date: 2021
  • Doi Number: 10.1002/elan.202100198
  • Journal Name: ELECTROANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Applied Science & Technology Source, BIOSIS, Chemical Abstracts Core, Chimica, Communication Abstracts, Metadex, Civil Engineering Abstracts
  • Page Numbers: pp.2078-2086
  • Keywords: Electrochemical Biosensor, mir-126, Voltammetry, Multi-walled Carbon Nanotubes, Gold Nanorods
  • Dokuz Eylül University Affiliated: No

Abstract

In this article, we introduced a novel electrochemical biosensor for the detection of microRNA-126. The biosensor utilizes a hybridization assay combined with multi-walled carbon nanotubes and gold nanorod-decorated screen-printed carbon electrodes. For electrode preparation, gold nanorods were first immobilized onto the surface of bare and multi-walled carbon nanotube-modified screen-printed carbon electrodes, and the thiol tagged-capture probe was immobilized on the electrode surface through gold and thiol group interaction. After the immobilization, thiol tagged-capture probe hybridized with the target sequence. Under optimum conditions, we determined limit of detection (LOD) and limit of quantification (LOQ) as high as 11 nM and 36 nM, respectively.