The main objectives of this study were to evaluate the health-related physical fitness for the patients with end-stage renal disease on hemodialysis and determine the suitability of Eurofit Test Battery for adults to decide their health-related physical fitness level. Eighteen patients with end-stage renal disease (ESRD) on hemodialysis (age 49.7 + 17.9, 10 female / 8 male) was evaluated and compared with 22 age-matched healthy subjects (age 50.5 + 9.4 years, 13 female / 9 male) for this purposes. Eurofit Test Battery for Adults is composed of aerobic fitness, musculoskeletal fitness, motor fitness, and anthropometry components. Aerobic fitness, which was assessed by six minutes walking distance, was lower in the patient group than controls (p < 0.05). Hemodialysis patients had lower motor fitness (0.000) and musculoskeletal fitness including vertical jump and handgrip tests (0.047, 0.002). Percentage of body fat and skinfold thickness values measured from triceps, subscapular, abdominal, and thigh were also lower in patient group (p < 0.05). Additionally no complication was seen during and/or after the tests. In conclusion, the Eurofit for adults may be considered a useful test battery to evaluate the physical fitness and design the health-related physical fitness program based on the Eurofit results in this population.