Akademik Veri Yönetim Sistemi
JOURNAL OF BASIC AND CLINICAL HEALTH SCIENCES, cilt.9, sa.1, 2025 (ESCI)
Purpose: The Epidermal Growth Factor Receptor (EGFR) gene and its associated pathways have recently emerged as promising targets in precision medicine for Non-Small Cell Lung Cancer (NSCLC). This study serves as a proof of concept, leveraging exosomal miRNAs as a cost-effective and minimally invasive liquid biopsy method. We aim to investigate four exosomal miRNAs: miR-22-3p, miR-221-3p, miR-30b and miR-30c and with miR-1288 serving as control. These miRNAs have been previously defined in the literature. Materials and Methods: A total of thirty-six samples from distinct Non-Small Cell Lung Cancer (NSCLC) cases are included. Exosomes are derived from the patients' plasma, followed by miRNA isolation, cDNA synthesis, and quantitative polymerase chain reactions. The triangle/triangle Ct approach is employed for quantification of miRNAs. Results: The controls: miRNA and miR-1288 are expressed in almost all samples. One sample is an exception. The two target miRNAs, miR-30c and miR-22-3p, generated successful polymerase chain reaction (PCR) curves. However, the remaining two miRNAs, miR-221-3p and miR-30b produced PCR curves with low amplitude. Conclusion: miR-30b, miR-30c, miR-221-3p, miR-22-3p have previously been reported to be associated with lung cancer, but they have not been studied in a patient series until now. In our study, exosomal miR-22-3p and miR-30c were isolated from the cancer patients' plasma, suggesting that they could serve as potential lung cancer biomarkers.