Akademik Veri Yönetim Sistemi
Journal of Electroanalytical Chemistry, cilt.1003, 2026 (SCI-Expanded, Scopus)
Morganella morganii is an emerging opportunistic pathogen associated with urinary tract infections, yet no selective biosensing platform has been reported for its rapid detection. In this study, we present the first molecularly imprinted polymer (MIP)-based electrochemical biosensor specifically tailored for M. morganii, enabling label-free, highly selective, and low-cost detection. The sensor was fabricated by electropolymerizing phenol in the presence of whole-cell bacteria on pencil graphite electrodes, followed by template removal to generate specific recognition cavities. Successful imprinting and selective rebinding were verified by electrochemical impedance spectroscopy and cyclic voltammetry. The biosensor demonstrated a low LOD of 3.0 CFU/mL in real urine, excellent linearity (R2 > 0.99), and high reproducibility (RSD < 10 %), confirming robust analytical performance. Notably, the sensor maintained high sensitivity in complex urine matrices, indicating strong matrix tolerance. Selectivity studies showed imprinting factors of 4.5–10.1 against E. coli, K. pneumoniae, S. aureus, and E. faecalis, confirming excellent discrimination capability. The biosensor retained >95 % of its initial response for 3 days and ∼ 56 % after 14 days, demonstrating satisfactory stability. Compared to conventional microbiological and molecular assays, this MIP-based platform offers a rapid and label-free strategy for early detection of M. morganii in urinary tract infection diagnostics.