Contractility of diabetic human corpus cavernosum smooth muscle in response to serotonin mediated via Rho-kinase.

Murat N., Soner B. C., Demir O., ESEN A. A., Gidener S.

Pharmacology, cilt.84, sa.1, ss.24-8, 2009 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 84 Sayı: 1
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1159/000221380
  • Dergi Adı: Pharmacology
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.24-8
  • Anahtar Kelimeler: Diabetes mellitus, Corpus cavernosum, human, Rho-kinase, Serotonin, ERECTILE, 5-HYDROXYTRYPTAMINE, DYSFUNCTION, MELLITUS
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Background/Aims: Serotonin (5-hydroxytryptamine, 5-HT)-induced contraction and the involvement of RhoA/Rho-kinase pathway in the 5-HT-induced contraction was investigated isometrically in vitro in both diabetic and nondiabetic human corpus cavernosum (HCC) tissues. Methods: HCC tissues were obtained from 12 patients. The response to 5-HT (10(-9) to 10(-5) mol/l) was studied in isolated HCC tissues in the absence and in the presence of a Rho-kinase inhibitor (Y-27632). Results: Preincubation with Y-27632 attenuated maximum contractions induced by 5-HT in tissues of both nondiabetics and diabetics. When diabetic and nondiabetic groups were compared, no significant difference was seen in 5-HT-induced contraction alone, but in the presence of Y-27632, 5-HT-induced contraction was significantly higher in the diabetic group. Conclusion: These results suggest that the Rho-kinase-mediated pathway plays an important role for 5-HT-induced contraction in diabetic corpus cavernosum tissues. Copyright (C) 2009 S. Karger AG, Basel