Accelerated electrochemical antibiotic susceptibility testing using disposable electrodes and Resazurin

Kaya H. O., Tekintas Y., Karadeniz H., Isbilir H., Temel A., TOPKAYA ÇETİN S. N.

Journal of Pharmaceutical and Biomedical Analysis, cilt.265, 2025 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 265
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1016/j.jpba.2025.117011
  • Dergi Adı: Journal of Pharmaceutical and Biomedical Analysis
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Analytical Abstracts, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, International Pharmaceutical Abstracts, Veterinary Science Database
  • Anahtar Kelimeler: Antibiotic susceptibility, Stenotrophomonas maltophilia, Shigella sonnei, Electrochemical sensors, Resazurin
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Stenotrophomonas maltophilia and Shigella sonnei are increasingly associated with serious infections and shigellosis, respectively, with conventional antibiotic susceptibility tests requiring 18–24 h or more, often delaying treatment. In this study, we present a novel electrochemical sensor employing the redox indicator Resazurin for rapid assessment of antibiotic susceptibility. Detection of live S. maltophilia and S. sonnei was achieved within 2 h as Resazurin, reduced to Resorufin by metabolically active bacteria, produced a measurable decrease in the reduction current. Following exposure to antibiotics (levofloxacin, trimethoprim-sulfamethoxazole, and ampicillin), changes in Resazurin's reduction current were monitored using Differential Pulse Voltammetry. Susceptible bacteria exhibited minimal changes in current, while resistant strains actively reduced Resazurin, lowering the current. This sensor provided results within 4–5 h, aligning with traditional methods like disk diffusion and broth microdilution but with improved speed and operational simplicity. These findings highlight the potential of the developed sensor as a rapid, reliable alternative for antibiotic susceptibility testing.