Gold nanoparticle probes: Design and in vitro applications in cancer cell culture


Unak G., Ozkaya F., MEDİNE E. İ., KOZGUŞ GÜLDÜ Ö., Sakarya S., BEKİŞ R., ...More

COLLOIDS AND SURFACES B-BIOINTERFACES, vol.90, pp.217-226, 2012 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 90
  • Publication Date: 2012
  • Doi Number: 10.1016/j.colsurfb.2011.10.027
  • Journal Name: COLLOIDS AND SURFACES B-BIOINTERFACES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.217-226
  • Keywords: Gold nanoparticles (AuNPs), F-18-FDG, Anti-metadherin (Anti-MTDH), Tumor imaging, Cancer diagnosis, MCF7, DRUG-DELIVERY, PROTEIN, RADIOTHERAPY
  • Dokuz Eylül University Affiliated: Yes

Abstract

A new architecture has been designed by the conjugation of [F-18]2-fluoro-2-deoxy-D-glucose (F-18-FDG), gold nanoparticles (AuNPs), and anti-metadherin (Anti-MTDH) antibody which is specific to the metadherin (MTDH) over-expressed on the surface of breast cancer cells. Mannose triflate molecule is used as a precursor for synthesis of F-18-FDG by nucleophilic fluorination. For the conjugation of F-18-FDG and AuNPs, cysteamine was first bound to mannose triflate (Man-CA) before synthesizing of F-18-FDG which has cysteamine sides ((18)FDG-CA). Then, (18)FDG-CA was reacted with HAuCl4 to obtain AuNPs and with NaBH4 for reduction of AuNPs. At the end of this procedure. AuNPs were conjugated to F-18-FDG via disulphide bonds ((18)FDG-AuNP). For the conjugation of Anti-MTDH, 1,1'-carbonyl diimidazol (CDI) was bound to the (18)FDG-AuNP, and Anti-MTDH was conjugated via CDI ((18)FDG-AuNP-Anti-MTDH). This procedure was also performed by using (NaF)-F-19 to obtain non-radioactive conjugates ((19)FDG-AuNP-Anti-MTDH). Scanning electron microscopy (SEM) images demonstrated that synthesized particles were in nano sizes. (18)FDG-AuNP-Anti-MTDH conjugate was characterized and used as a model probe containing both radioactive and optical labels together as well as the biological target. The (18)FDG-AuNP-Anti-MTDH conjugate was applied to MCF7 breast cancer cell line and apoptotic cell ratio was found to be increasing from 2% to 20% following the treatment. Hence, these results have promised an important application potential of this conjugate in cancer research. (C) 2011 Elsevier B.V. All rights reserved.