TESCALCIN OVER-EXPRESSION INCREASES KERATIN 18 AND 19 IN K562 CELLS

Efe H., Yoca O. U., Onkol U. O., YÜCE Z.

JOURNAL OF BASIC AND CLINICAL HEALTH SCIENCES, cilt.6, sa.3, ss.858-862, 2022 (ESCI) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 6 Sayı: 3
  • Basım Tarihi: 2022
  • Doi Numarası: 10.30621/jbachs.1101524
  • Dergi Adı: JOURNAL OF BASIC AND CLINICAL HEALTH SCIENCES
  • Derginin Tarandığı İndeksler: Emerging Sources Citation Index (ESCI)
  • Sayfa Sayıları: ss.858-862
  • Anahtar Kelimeler: Keratins, TESC, K562
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

Purpose: Tescalcin (TESC) has been shown to be essential in mammalian cells as a regulator of intracellular Ca2+. Ca2+ is a second messenger functioning in many metabolic pathways as well as in cell differentiation, cell size and the cell cycle. K562 cells over-expressing TESC change their morphology and adopt adherent properties. Considering differences in morphology may have been reflected in changes of the cytoskeleton, we focused on the expression levels of keratins, which are cytoskeletal intermediate filaments in epithelial cells and also expressed in K562. We over-expressed the TESC gene via lentiviral transduction and analyzed keratin 8 (K8), keratin 18 (K18), and keratin 19 (K19) expression.