TESCALCIN OVER-EXPRESSION INCREASES KERATIN 18 AND 19 IN K562 CELLS


Efe H., Yoca O. U., Onkol U. O., YÜCE Z.

JOURNAL OF BASIC AND CLINICAL HEALTH SCIENCES, vol.6, no.3, pp.858-862, 2022 (ESCI) identifier

Abstract

Purpose: Tescalcin (TESC) has been shown to be essential in mammalian cells as a regulator of intracellular Ca2+. Ca2+ is a second messenger functioning in many metabolic pathways as well as in cell differentiation, cell size and the cell cycle. K562 cells over-expressing TESC change their morphology and adopt adherent properties. Considering differences in morphology may have been reflected in changes of the cytoskeleton, we focused on the expression levels of keratins, which are cytoskeletal intermediate filaments in epithelial cells and also expressed in K562. We over-expressed the TESC gene via lentiviral transduction and analyzed keratin 8 (K8), keratin 18 (K18), and keratin 19 (K19) expression.