Effects of ozone and photo-activated disinfection against Enterococcus faecalis biofilms in vitro


GÜÇLÜER Ö., Dincer A. N., KUŞTARCI A., ER Ö., DİNÇ G., DEMİRBUĞA S.

NIGERIAN JOURNAL OF CLINICAL PRACTICE, cilt.18, sa.6, ss.814-818, 2015 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 18 Sayı: 6
  • Basım Tarihi: 2015
  • Doi Numarası: 10.4103/1119-3077.163289
  • Dergi Adı: NIGERIAN JOURNAL OF CLINICAL PRACTICE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.814-818
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

Objectives: The purpose of this study was to compare the antibacterial effects of gaseous ozone (O-3) and photo-activated disinfection (PAD) methods against Enterococcus faecalis (E. faecalis) biofilms. Materials and Methods: Sixty-five human mandibular premolars with straight root canals were selected. After root canal preparation, the samples were sterilized and placed into eppendorf tubes with 1 mL brain heart infusion broth containing 1.5 x 10(8) colony-forming units (CFUs)/mL of E. faecalis. The contaminated samples were then divided into four groups (n = 15) according to the disinfection method used: Group 1, Saline (positive control); Group 2, NaOCl (negative control); Group 3, Gaseous O-3; and Group 4, PAD. Three non-contaminated teeth were used to control the infection and sterilization process. The CFUs were counted and the data were analyzed statistically. Results: There was a statistically significant difference between the experimental and control groups (P < 0.05). The saline group had the highest number of remaining microorganisms. Complete sterilization was achieved in the 2.5% NaOCl group. There were no statistically differences between PAD and gaseous O-3 (P > 0.05). Conclusion: Both PAD and gaseous O-3 have a significant antibacterial effect on infected root canals. However, 2.5% NaOCl was superior in terms of its antimicrobial abilities compared with the other disinfection procedures.