Human Papilloma Virus-11 DNA Detection by Graphene-PAMAM Modified Impedimetric CRISPR-dCas9 Biosensor

Mumcu M. U., ERTUĞRUL UYGUN H. D., UYGUN Z. O.

ELECTROANALYSIS, vol.34, no.5, pp.830-834, 2022 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 34 Issue: 5
  • Publication Date: 2022
  • Doi Number: 10.1002/elan.202100536
  • Journal Name: ELECTROANALYSIS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Applied Science & Technology Source, BIOSIS, Chemical Abstracts Core, Chimica, Communication Abstracts, Metadex, Civil Engineering Abstracts
  • Page Numbers: pp.830-834
  • Keywords: biosensor, CRISPR, dCas9, HPV-11, chronoimpedance
  • Dokuz Eylül University Affiliated: Yes

Abstract

Human Papilloma Virus-11 (HPV-11) is leads to condylomata acuminata (CA), which has commonly known as genital wards that global widespread incidence of 160 to 289 cases per year. In the first-time literature, we detect HPV-11 DNA by using dCas9 modified graphene oxide-PAMAM modified electrodes, impedimetrically. Chronoimpedimetric detection was facilitated the biosensor response time optimization of HPV-11 DNA in 5 minutes. The biosensor has ability to analyze HPV-11 DNA between 50 pM and 1000 pM with good linearity, sensitivity and selectivity. Moreover, we tested our biosensor in real samples matrix by considering recovery of the samples.