Development of Fluorescent Array Based on Sol-Gel/Chitosan Encapsulated Acetylcholinesterase and pH Sensitive Oxazol-5-one Derivative

Ozturk G., Feller K., Dornbusch K., TİMUR S., ALP S., Ergun Y.

JOURNAL OF FLUORESCENCE, cilt.21, sa.1, ss.161-167, 2011 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 21 Sayı: 1
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1007/s10895-010-0700-7
  • Dergi Adı: JOURNAL OF FLUORESCENCE
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.161-167
  • Anahtar Kelimeler: Fluorescent array, Sol-gel, Chitosan, Acetylcholinesterase, Acetylcholine, Biosensor, PYRIDOSTIGMINE BROMIDE, BIOSENSOR, IMMOBILIZATION, CORTICOSTERONE, STRESS, SYSTEM, PVC
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

A highly sensitive fluorescent enzyme array for quantitative acetylcholine detection is developed. The enzyme array has been constructed by spotting of pH sensitive fluorophore 2-phenyl-4-[4-(1,4,7,10-tetraoxa-13-azacycloopentadecyl)benzylidene]oxazol-5-one and acetylcholinesterase doped in tetraethoxysilane / chitosan matrix via a microarrayer. The constructed tetraethoxysilane / chitosan network provided a microenvironment in which the enzyme molecule was active biologically. The optimal operational conditions for the array developed were investigated. The response of the developed biosensor array to acetylcholine was highly reproducible (RSD = 3.27%, n = 6). A good linearity was observed for acetylcholine in the concentrations up to 1 x 10(-8) M, with a detection limit of 0.27 x 10(-8) M.