Dark fermentative bio-hydrogen production from waste wheat starch using co-culture with periodic feeding: Effects of substrate loading rate

ÖZMIHÇI S., Kargi F.

INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, vol.36, no.12, pp.7089-7093, 2011 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 36 Issue: 12
  • Publication Date: 2011
  • Doi Number: 10.1016/j.ijhydene.2011.03.071
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.7089-7093
  • Keywords: Bio-hydrogen, Dark fermentation, Ground wheat starch, Periodic feeding, Substrate loading rate, CONTINUOUS BIOHYDROGEN PRODUCTION, PERFORMANCE, CULTURE, BATCH
  • Dokuz Eylül University Affiliated: Yes


Dark fermentation experiments were performed for bio-hydrogen production from ground wheat starch solution (10 +/- 1 g l(-1)) using periodic feeding and effluent removal. A mixed culture of Clostridium butyricum-NRRL 1024 and Clostridium pasteurianum-NRRL B-598 were used with an initial biomass ratio of 1/1. Effects of wheat starch loading rate on the rate and yield of bio-hydrogen formation were investigated. Substrate loading rate was varied between 0.54 and 5.52 g d(-1) (HRT = 6-60 h). The highest hydrogen formation rate (280 ml d(-1)), volumetric hydrogen formation rate (1857 ml H-2 l(-1) d(-1)) and volatile fatty acids (VFAs) concentration were obtained with a substrate loading rate of 5.52 g d(-1) (HRT = 6 h). The highest hydrogen yield (109 ml H-2 g TS-1) was obtained with a substrate loading rate of 1.38 g d(-1). Copyright (C) 2011, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.