Lipopolysaccharide-preconditioning protects against endotoxin-induced white matter injury in the neonatal rat brain.

KUMRAL A., Tuzun F., ÖZBAL S., Ergur B. U., YILMAZ O., DUMAN N., ...More

Brain research, vol.1489, pp.81-9, 2012 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 1489
  • Publication Date: 2012
  • Doi Number: 10.1016/j.brainres.2012.10.015
  • Journal Name: Brain research
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.81-9
  • Keywords: Newborn, Periventricular leukomalacia, Tolerance, Antioxidant, SOD, Preconditioning, Apoptosis, Hypomyelination, White matter injury, Rat, FOCAL CEREBRAL-ISCHEMIA, NECROSIS-FACTOR-ALPHA, SUPEROXIDE-DISMUTASE, TOLERANCE, EXPRESSION, INFLAMMATION, CHORIOAMNIONITIS, RESISTANCE, INDUCTION, INFANTS
  • Dokuz Eylül University Affiliated: Yes


Background: Exposing the brain to a sub-damaging stimulus can protect against a subsequent lethal insult, a phenomenon termed preconditioning. The aim of this study was to investigate the neuroprotective effect of low dose LPS (lipopolysaccharide) pretreatment in endotoxin induced periventricular leukomalacia (PVL) in a rat model. Methods: Wistar rats with dated pregnancies were allocated to 5 groups: (i) no LPS administered, intraperitoneally (i.p.) pyrogen-free saline injected (Control group), (ii) 500 mu g/kg LPS administrated i.p. on days 18 and 19 (PVL group), (iii) 50 mu g/kg LPS administrated i.p. on day 17 followed by 500 mu g/kg LPS i.p. on days 18 and 19 (PC-PVL group), (iv) 50 mu g/kg LPS administrated on day 17 (PC only), and (v) i.p. pyrogen-free saline injected control group on day 17. Results: LPS-preconditioning given 24 h before potent LPS exposure significantly reduced the number of apoptotic cell deaths and prevented hypomyelination. Antioxidant enzyme gene expression levels (Superoxide Dismutase-SOD1, SOD2, and SOD3) were increased and Tumor Necrosis Factor (TNF)alpha expression levels were decreased in the PC+PVL group when compared with the PVL group. Conclusion: Low-dose LPS given one day before potent doses of LPS reduces antepartum LPS-induced brain damage. The mechanisms of protection might involve oxidation and inflammation. (C) 2012 Elsevier B.V. All rights reserved.