Interferon gamma and lipopolysaccharide upregulate TNF-related apoptosis-inducing ligand expression in murine microglia

Genc Ş., Kizyldag S., Genc K., Ateş H., Atabey N.

IMMUNOLOGY LETTERS, cilt.85, sa.3, ss.271-274, 2003 (SCI-Expanded) identifier identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 85 Sayı: 3
  • Basım Tarihi: 2003
  • Doi Numarası: 10.1016/s0165-2478(02)00245-6
  • Dergi Adı: IMMUNOLOGY LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.271-274
  • Anahtar Kelimeler: TRAIL, microglia, N9 cell line, murine, neurodegeneration, TUMOR-NECROSIS-FACTOR, TRAIL, CELLS, INDUCTION, BRAIN
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

In this study, it is reported that neonatal murine microglia and N9 murine microglial cell line express tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL). TRAIL protein and mRNA expression in murine microglia greatly upregulate upon stimulation with interferon gamma (IFNgamma) or lipopolysaccharide (LPS) as revealed by immunoprecipitation-immunoblotting, reverse transcriptase-polymerase chain reaction (RT-PCR) and flow cytometry techniques. IFNgamma and LPS act synergistically to induce TRAIL expression on both translational and transcriptional levels. The upregulated microglial TRAIL in inflammatory conditions may involve in the cytotoxic effect of these cells and play a role in neurodegenerative processes. (C) 2002 Elsevier Science B.V. All rights reserved.