Interferon gamma and lipopolysaccharide upregulate TNF-related apoptosis-inducing ligand expression in murine microglia

Genc Ş., Kizyldag S., Genc K., Ateş H., Atabey N.

IMMUNOLOGY LETTERS, vol.85, no.3, pp.271-274, 2003 (SCI-Expanded) identifier identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 85 Issue: 3
  • Publication Date: 2003
  • Doi Number: 10.1016/s0165-2478(02)00245-6
  • Journal Name: IMMUNOLOGY LETTERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.271-274
  • Keywords: TRAIL, microglia, N9 cell line, murine, neurodegeneration, TUMOR-NECROSIS-FACTOR, TRAIL, CELLS, INDUCTION, BRAIN
  • Dokuz Eylül University Affiliated: Yes

Abstract

In this study, it is reported that neonatal murine microglia and N9 murine microglial cell line express tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL). TRAIL protein and mRNA expression in murine microglia greatly upregulate upon stimulation with interferon gamma (IFNgamma) or lipopolysaccharide (LPS) as revealed by immunoprecipitation-immunoblotting, reverse transcriptase-polymerase chain reaction (RT-PCR) and flow cytometry techniques. IFNgamma and LPS act synergistically to induce TRAIL expression on both translational and transcriptional levels. The upregulated microglial TRAIL in inflammatory conditions may involve in the cytotoxic effect of these cells and play a role in neurodegenerative processes. (C) 2002 Elsevier Science B.V. All rights reserved.