Surface plasmon resonance aptasensor for soluble ICAM-1 protein in blood samples

Dursun A. D., Dogan S., Kavruk M., Busra Tasbasi B., Sudagidan M., Deniz Yilmaz M., ...More

Analyst, vol.147, no.8, pp.1663-1668, 2022 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 147 Issue: 8
  • Publication Date: 2022
  • Doi Number: 10.1039/d1an02332b
  • Journal Name: Analyst
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, Aerospace Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, MEDLINE, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Page Numbers: pp.1663-1668
  • Dokuz Eylül University Affiliated: No

Abstract

Intercellular Adhesion Molecule-1 (ICAM-1) is considered to be a cancer biomarker in the assessment of metastatic potential in patients and an early indicator of atherosclerosis. A labelless biosensor based on the surface plasmon resonance (SPR) signal from the specific affinity interaction of an aptamer and a soluble ICAM-1 protein was developed for blood samples. The developed aptasensor provided real-time information on the concentration of the ICAM-1 protein in blood when integrated to a purification step based on a magnetic pull-down separation. The SPR aptasensor was highly specific with a limit of detection of 1.4/0.2 ng ml−1, which was achieved through aptamer-functionalized silica-coated magnetic nanoparticles.