Molecular detection of bcr/abl m-RNA and cytogenetic analysis in CML patients: Comparison of results and factors affecting the methodologies used
Turkish Journal of Cancer, cilt.28, sa.4, ss.166-173, 1998 (Scopus, TRDizin)
- Yayın Türü: Makale / Tam Makale
- Cilt numarası: 28 Sayı: 4
- Basım Tarihi: 1998
- Dergi Adı: Turkish Journal of Cancer
- Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
- Sayfa Sayıları: ss.166-173
- Anahtar Kelimeler: BCR/ABL, Chronic myeloid leukemia, Cytogenetic analysis, RT-PCR
- Dokuz Eylül Üniversitesi Adresli: Evet
Özet
The Philadelphia chromosome (Ph) is the cytogenetic hallmark of chronic myeloid leukemia (CML), based on clinical and morphological criteria. Both cytogenetic analysis and reverse transcriptase-PCR (RT-PCR) detection of the chimeric bcr/abl mRNAs were performed on 30 patients prediagnosed as CML. The methodology and results of RT-PCR detection of the bcr/abl mRNA, and those of cytogenetic analysis are compared and discussed. Our results emphasize the need of both procedures in the diagnosis and follow-up of CML patients.