Molecular detection of bcr/abl m-RNA and cytogenetic analysis in CML patients: Comparison of results and factors affecting the methodologies used

SERCAN H. O., Eresen C., Yuksei E., Sercan Z., PARALI F., ALTUNGÖZ O., ...Daha Fazla

Turkish Journal of Cancer, cilt.28, sa.4, ss.166-173, 1998 (Scopus) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 28 Sayı: 4
  • Basım Tarihi: 1998
  • Dergi Adı: Turkish Journal of Cancer
  • Derginin Tarandığı İndeksler: Scopus, TR DİZİN (ULAKBİM)
  • Sayfa Sayıları: ss.166-173
  • Anahtar Kelimeler: BCR/ABL, Chronic myeloid leukemia, Cytogenetic analysis, RT-PCR
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

The Philadelphia chromosome (Ph) is the cytogenetic hallmark of chronic myeloid leukemia (CML), based on clinical and morphological criteria. Both cytogenetic analysis and reverse transcriptase-PCR (RT-PCR) detection of the chimeric bcr/abl mRNAs were performed on 30 patients prediagnosed as CML. The methodology and results of RT-PCR detection of the bcr/abl mRNA, and those of cytogenetic analysis are compared and discussed. Our results emphasize the need of both procedures in the diagnosis and follow-up of CML patients.