Effect of boramidic acid modified carbon nanotubes on neurological, morphological and physiological responses of zebrafish (Danio rerio) embryos and larvae

Yiğit A., Köktürk M., YILDIRIM S., Nazlı D., Kılıççıoğlu M., Şahin A., ...More

Science of the Total Environment, vol.947, 2024 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 947
  • Publication Date: 2024
  • Doi Number: 10.1016/j.scitotenv.2024.174614
  • Journal Name: Science of the Total Environment
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Analytical Abstracts, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, Environment Index, Food Science & Technology Abstracts, Geobase, Greenfile, Metadex, Pollution Abstracts, Veterinary Science Database, Civil Engineering Abstracts
  • Keywords: Apoptosis, Carbon nanoparticles, Danio rerio, DNA damage, Morphological abnormality
  • Dokuz Eylül University Affiliated: Yes

Abstract

This study aimed to determine the potential toxicological effects of carbon nanotubes (CNTs), their modifications with ethylenediamine (ED) and boric acid (BA) on aquatic organisms. Specifically, the research focused on the morphological, physiological, and histopathological-immuno-histochemical responses in zebrafish (Danio rerio) embryos and larvae, via applying different concentrations of CNTs, CNT-ED, and CNT-ED-BA (Control, 5, 10, and 20 mg/L). The results indicated that 20 mg/L CNT nanoparticles were toxic to zebrafish larvae, with mortality rates increasing with CNT and CNT-ED concentrations, reaching 36.7 % at the highest CNT concentration. The highest dose caused considerable degeneration, necrosis, DNA damage, and apoptosis, as evidenced by histopathological and immunohistochemical tests. In contrast, despite their high concentration, CNT-ED-BA nanoparticles exhibited low toxicity. Behavioral studies revealed that CNT and CNT-ED nanoparticles had a more significant impact on sensory-motor functions compared to CNT-ED-BA nanoparticles. These findings suggest that modifying the nanosurface with boric acid, resulting in boramidic acid, can reduce the toxicity induced by CNT and CNT-ED.