Development of a new biosensor for mediatorless voltammetric determination of hydrogen peroxide and its application in milk samples

Alpat Ş., Kılınç Alpat S., Dursun Z., Telefoncu A.

JOURNAL OF APPLIED ELECTROCHEMISTRY, cilt.39, sa.7, ss.971-977, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 39 Sayı: 7
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1007/s10800-009-9776-7
  • Dergi Adı: JOURNAL OF APPLIED ELECTROCHEMISTRY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.971-977
  • Anahtar Kelimeler: Hydrogen peroxide, Voltammetry, Biosensor, Clinoptilolite, Catalase, Milk, CARBON-PASTE ELECTRODE, HORSERADISH-PEROXIDASE, AMPEROMETRIC DETERMINATION, ENZYME ELECTRODE, METHYLENE-BLUE, SENSOR, IMMOBILIZATION, FILM, COIMMOBILIZATION, FABRICATION
  • Dokuz Eylül Üniversitesi Adresli: Evet

Özet

A new biosensor for the voltammetric detection of hydrogen peroxide was developed based on immobilization of catalase on a clinoptilolite modified carbon paste electrode using bovine serum albumin and glutaraldehyde. The biosensor response was evaluated according to electrode composition, reaction time, solution pH and temperature. The voltammetric signals were linearly in proportion to H2O2 concentration in the range 5.0 x 10(-6) - 1.0 x 10(-3) M with a correlation coefficient of 0.9975. The detection limit is 8.0 x 10(-7) M and the relative standard deviation for 4.0 x 10(-4) M hydrogen peroxide was 1.83% (n = 6). The biosensor exhibited high sensitivity, and it was determined that it could be used for more than 2 months. In addition, the biosensor was successfully applied for the determination of hydrogen peroxide in milk samples.