The Effects of Interferon alpha 2b on Chemically-induced Peritoneal Fibrosis and on Peritoneal Tissue MMP-2 and TIMP-2 Levels in Rats

Ucar E., Borazan A., Semerci E., Binici D. N., Yaldiz M., AYSAL AĞALAR A., ...More

JOURNAL OF INTERNATIONAL MEDICAL RESEARCH, vol.38, no.1, pp.187-194, 2010 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 38 Issue: 1
  • Publication Date: 2010
  • Doi Number: 10.1177/147323001003800121
  • Journal Name: JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.187-194
  • Keywords: PERITONEAL FIBROSIS, INTERFERON-alpha 2B (IFN-alpha 2B), CHLORHEXIDINE GLUCONATE, RATS, MATRIX METALLOPROTEINASE-2 (MMP-2), TISSUE INHIBITORS METALLOPROTEINASE-2 (TIMP-2), PARIETAL THICKENING, MATRIX METALLOPROTEINASES, DIALYSIS, DISEASE, INJURY, MARKER, MODEL
  • Dokuz Eylül University Affiliated: Yes

Abstract

This study investigated the effect of interferon alpha 2b on chlorhexidine gluconate (CH)-induced peritoneal fibrosis (PF) in rats and assessed peritoneal tissue levels of metalloproteinase (MMP)-2 and tissue inhibitors of metalloproteinases (TIMP)-2. Wistar albino rats (n = 8 per group) were treated as follows: control group, 3 ml/day of 0.9% saline intraperitoneally for 28 days; CH group, 0.1% CH (200 g [3 ml]/day) in 15% ethanol and 0.9% saline intra-peritoneally for 28 days; CH + interferon (IFN) group, CH (as above) plus pegylated IFN-alpha 2b 1.5 mu g/kg per week subcutaneously on days 0, 7, 14, 21 and 28; IFN group, pegylated IFN-alpha 2b (as above). Parietal peritoneum samples were obtained from the left anterior abdominal wall after 35 days. Parietal thickness, degree of vascular proliferation and inflammation, and MMP-2 and TIMP-2 levels were determined. The mean peritoneal thicknesses of the control, CH, CH + IFN and IFN groups were 7.02 +/- 3.89, 156.86 +/- 29.13, 59.88 +/- 22.1, 9.27 +/- 2.03 mu m, respectively. Pegylated IFN-alpha 2b decreased CH-induced expression of MMP-2 in the parietal peritoneum, but had no effect on TIMP-2 levels. Further studies are needed to determine the optimal dosage and duration for pegylated IFN-alpha 2b treatment.