This study aims to reveal to what extent Turkish chemistry teachers use laboratorys effectively and their perceptions on laboratory applications and the factors related to laboratory applications. In this cross-sectional survey, 408 chemistry teachers from the secondary schools in Izmir were given "Teacher Demographic form", "The Scale of Chemistry teachers' perceptions on Laboratory Applications" and "The Questionnaire for the factors effecting Laboratory Applications". The findings revealed that the teachers' perceptions on laboratory applications significantly vary according to their self-efficacy beliefs in laboratory applications, the type of school they graduated, the type of the school they are working at, their experience in teaching, their schools' physical conditions and assesment-evaluation techniques and chemistry program. The type of the experiment they use and the type of their schools are significantly correlated. However, their experiences in teaching, the type of school they graduated, the type of experiment they use are significantly uncorrelated. The teachers from Anatolian high schools mostly choose open-ended experiments while the others form vocational and state high schools choose closed end experiments. It is quite remarkable that all teachers in the survey prefer hypothesis-based experiments.