Fed-batch fermentation of GM-CSF-producing glycoengineered <i>Pichia pastoris</i> under controlled specific growth rate

Jacobs P. P., İNAN M., Festjens N., Haustraete J., Van Hecke A., Contreras R., ...More

MICROBIAL CELL FACTORIES, vol.9, 2010 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 9
  • Publication Date: 2010
  • Doi Number: 10.1186/1475-2859-9-93
  • Journal Name: MICROBIAL CELL FACTORIES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Dokuz Eylül University Affiliated: No

Abstract

Background: Yeast expression systems with altered N-glycosylation are now available to produce glycoproteins with homogenous, defined N-glycans. However, data on the behaviour of these strains in high cell density cultivation are scarce. Results: Here, we report on cultivations under controlled specific growth rate of a GlycoSwitch-Man5 Pichia pastoris strain producing Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) at high levels (hundreds of milligrams per liter). We demonstrate that homogenous Man(5)GlcNAc(2) N-glycosylation of the secreted proteins is achieved at all specific growth rates tested. Conclusions: Together, these data illustrate that the GlycoSwitch-Man5 P. pastoris is a robust production strain for homogenously N-glycosylated proteins.