Journal of Microbiological Methods, cilt.244, 2026 (SCI-Expanded, Scopus)
The study aims to evaluate the effectiveness of Mitis Salivarius Agar (MSA) media for the growth of two fungi belonging to the genus Geotrichum in comparison to conventional fungal medium. Potato dextrose agar (PDA), Sabouraud dextrose agar (SDA), and Rose Bengal chloramphenicol agar (RBC) were the standard media used in the comparative investigation. The two species' optimal development, the rate of culture growth, and their sizes, shapes, and textures on the four media were evaluated at two different temperature settings (20 °C–25 °C and 37 °C). Our results revealed that Mitis Salivarius Agar was remarkably effective in cultivating Geotrichum candidum and Geotrichum silvicola in contrast to the traditional media. Both species displayed a distinct colony on MSA after 15 days at ambient temperature (20–25 °C). After 15 days at 20–25 °C, Geotrichum candidum and Geotrichum silvicola exhibited a well-defined colony with the average growth diameters that were approximately close to the four media, with a little advantage of PDA and SDA. After being incubated at 37 °C, the two species' development on the four media was significantly ill-defined. The findings and statistical analysis indicated that Mitis Salivarius Agar provided a competitive medium for the growth of Geotrichum candidum and Geotrichum silvicola when compared to the three common fungal media (PDA, SDA, and RBC). This work may promote the successful use of this product as a fungal medium for filamentous fungi and encourage testing against other fungi.