Biological markers in cerebrospinal fluid (CSF) and evaluation of in vitro effect of CSF on PC12 cell line viability in Alzheimer's disease

YAKA E., YÜKSEL EĞRİLMEZ M., Keskinoglu P., ÇAVDAR Z., GENÇ Ş., Genc K., ...More

CELL BIOCHEMISTRY AND FUNCTION, vol.27, no.6, pp.395-401, 2009 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 27 Issue: 6
  • Publication Date: 2009
  • Doi Number: 10.1002/cbf.1588
  • Journal Name: CELL BIOCHEMISTRY AND FUNCTION
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.395-401
  • Keywords: Alzheimer's disease, CSF markers, cytotoxicity, PC12 cell line, tau, PHOSPHORYLATED-TAU, SENILE-DEMENTIA, NEURONS, CULTURE, BETA-AMYLOID((1-42)), A-BETA-1-42(43), SURVIVAL, PROTEIN, GROWTH, BRAIN
  • Dokuz Eylül University Affiliated: Yes

Abstract

The definite diagnosis of Alzheimer's disease (AD) is based on post mortem pathological examination. To date, there is no laboratory test that can discriminate AD patients from healthy individuals. In the perspective of recent knowledge, there are three cerebrospinal fluid (CSF) markers which have the highest sensitivity and specificity: A beta(1-40), A beta(1-42), and p-tau. In the. present study, 15 'Probable Alzheimer's Disease' (PAD) patients and 15 control subjects were included. PAD patients were selected from the patients of Dokuz Eylul University Neurology Department Dementia Outpatient clinic and control subjects were selected from the patients who were undergone epidural anesthesia because of any surgical operation. The concentrations of Ab1-40, Ab1-42, and p-tau in CSF were quantified by using ELISA. Also, the effects of 'PAD patients' CSF on the Survival of PC 12 cell line were assessed. There was a significant decrease of Ab1-40 and increase of p-tau in patients with AD when compared with controls. Ab1-42 concentration was not significantly different between groups. There was a positive corelation between duration of the disease and CSF of p-tau concentration in patients with AD. There was no significant difference in cell line viability Values between groups. Copyright (C) 2009 John Wiley & Sons, Ltd.