Research Information System
CATTLE PRACTICE, vol.30, no.12, pp.1-18, 2022 (SCI-Expanded)
Bovine mastitis is a multifactorial
disease that causes economic losses in the dairy industry on a global scale. n
this study, the presence of Trueperella
pyogenes and antibiotic resistance (ermX,
ermB and tetW) and virulence factor were determined in 150 milk samples
collected from cows of different breeds and ages with clinical mastitis in
dairy farms located in İzmir province and its districts (Bayindir, Beydağı,
Kiraz, Ödemiş, Tire). It was aimed to detect genes (plo, nanH, nanP, cbpA, fimA, fimC, fimE and fimG). The 17
of T.
pyogenes strains (11.3%) were isolated from a total of 150 samples by
culture and biochemical tests from milk samples. 4 (23.5%) were evaluated as
pure cultures, the other 13 (76.5%) isolates were obtained from mixed cultures.
The virulence genes of plo, nanH, nanP and fimA were
determined in all isolates. FimC, fimE, cbpA, fimG genes were
detected in 13 (76.4%), 12 (70.5%), 12 (70.5%) and 11 (64.7%) isolates,
respectively. Eight isolates had all 8 genes encoding virulence factors. In
contrast, 2 isolates carried the fimA,
plo, nanH and nanP genes.
Macrolide resistance genes ermX and ermB were detected in 14 (82.3%) and 9
(52.9%) isolates, respectively. The tetracycline resistance gene tetW was not detected in all isolates.
In the study, complete resistance was found for ampicillin, cefotaxime, and
penicillin G (17/17; 100%), followed by kanamycin and tetracycline (9/17; 53%),
respectively. Sensitivity was determined for trimethoprim-sulfamethoxazole with
the highest rate of 53% (9/17). As a result, clear evidence was obtained that
the virulence factors and antibiotic resistance of Trueperella pyogenes agents play a role in bovine mastitis and
various other infections.