Carbonic anhydrase activators: Activation of the beta-carbonic anhydrase Nce103 from the yeast Saccharomyces cerevisiae with amines and amino acids

Isik S., Kockar F., Aydin M., Arslan O., Guler O. O., Innocenti A., ...More

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, vol.19, no.6, pp.1662-1665, 2009 (SCI-Expanded, Scopus) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 6
  • Publication Date: 2009
  • Doi Number: 10.1016/j.bmcl.2009.01.105
  • Journal Name: BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1662-1665
  • Keywords: Carbonic anhydrase, Beta-class enzyme, Saccharomyces cerevisiae, Candida albicans, Cryptococcus neoformans, Amine, Amino acid, Enzyme mechanism
  • Dokuz Eylül University Affiliated: No

Abstract

The protein encoded by the Nce103 gene of Saccharomyces cerevisiae, a beta-carbonic anhydrase (CA, EC 4.2.1.1) designated as scCA, was investigated for its activation with amines and amino acids. scCA was poorly activated by amino acids such as L-/D-His, Phe, DOPA, Trp (K(A)s of 82 - 90 mu M) and more effectively activated by amines such as histamine, dopamine, serotonin, pyridyl-alkylamines, aminoethyl-piperazine/morpholine (K(A)s of 10.2-21.3 mu M). The best activator was L-adrenaline, with an activation constant of 0.95 mu M. This study may help to better understand the catalytic/activation mechanisms of the beta-CAs and eventually to design modulators of CA activity for similar enzymes present in pathogenic fungi, such as Candida albicans and Cryptococcus neoformans. (C) 2009 Elsevier Ltd. All rights reserved.