Carbonic anhydrase activators: Activation of the beta-carbonic anhydrase Nce103 from the yeast Saccharomyces cerevisiae with amines and amino acids

Isik S., Kockar F., Aydin M., Arslan O., Guler O. O., Innocenti A., ...Daha Fazla

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, cilt.19, sa.6, ss.1662-1665, 2009 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 19 Sayı: 6
  • Basım Tarihi: 2009
  • Doi Numarası: 10.1016/j.bmcl.2009.01.105
  • Dergi Adı: BIOORGANIC & MEDICINAL CHEMISTRY LETTERS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.1662-1665
  • Anahtar Kelimeler: Carbonic anhydrase, Beta-class enzyme, Saccharomyces cerevisiae, Candida albicans, Cryptococcus neoformans, Amine, Amino acid, Enzyme mechanism, ISOZYME-IV ACTIVATORS, ACTIVE-SITE, CRYSTALLOGRAPHIC ANALYSIS, CLASS ENZYME, ISOFORM-VII, GENE NCE103, INHIBITORS, VA, XIV, NEOFORMANS
  • Dokuz Eylül Üniversitesi Adresli: Hayır

Özet

The protein encoded by the Nce103 gene of Saccharomyces cerevisiae, a beta-carbonic anhydrase (CA, EC 4.2.1.1) designated as scCA, was investigated for its activation with amines and amino acids. scCA was poorly activated by amino acids such as L-/D-His, Phe, DOPA, Trp (K(A)s of 82 - 90 mu M) and more effectively activated by amines such as histamine, dopamine, serotonin, pyridyl-alkylamines, aminoethyl-piperazine/morpholine (K(A)s of 10.2-21.3 mu M). The best activator was L-adrenaline, with an activation constant of 0.95 mu M. This study may help to better understand the catalytic/activation mechanisms of the beta-CAs and eventually to design modulators of CA activity for similar enzymes present in pathogenic fungi, such as Candida albicans and Cryptococcus neoformans. (C) 2009 Elsevier Ltd. All rights reserved.