Erythropoietin decreases apoptosis induced by 6-hydroxydopamine in PC12 cells

Genç Ş., Ay M. E., Ateş H.

Turk Geriatri Dergisi, vol.9, no.1, pp.13-18, 2006 (SCI-Expanded) identifier

  • Publication Type: Article / Article
  • Volume: 9 Issue: 1
  • Publication Date: 2006
  • Journal Name: Turk Geriatri Dergisi
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, TR DİZİN (ULAKBİM)
  • Page Numbers: pp.13-18
  • Keywords: 6-hydroxydopamine, Apoptosis, CREB, DP5, Erythropoietin, Parkinson's desease, PC12 cell line
  • Dokuz Eylül University Affiliated: Yes

Abstract

Aim: In the present study, we investigated whether Erythropoietin (Epo) has a protective effect against cytotoxicity and apoptotic cell death induced by 6-hydroxydopamine (6-OHDA) in PC12 cell line cultures. Material and Methods: Epo exerted significant protective effect against 6-OHDA-induced cell injury and apoptosis as confirmed by lactate dehydrogenase cytotoxicity assay, anti-single strand DNA immunostaining and annexin-V staining. Results: Epo significantly decreased 6-OHDA-induced DP5 pro-apoptotic protein mRNA expression and increased bcl-XL anti-apoptotic protein mRNA expression. Any effect of Epo on CREB transcription factor phosphorylation was not observed. Conclusion: These results suggest that Epo exerts neuroprotective effect against 6-OHDA-induced cell death at least partially via the differential regulation of the expression of genes involved in the apoptotic process.